Application Note - Intrinsic Viscosity Analysis of Calf Thymus DNA

DNA (deoxyribonucleic acid) encodes for the genetic information of living organisms and is crucial in the cell division process. There is an interest in finding a reliable and convenient method to calculating the molecular weight of DNA. Intrinsic viscosity analysis is a reliable and convenient method to calculating the molecular weight of DNA. 

In this application note, we show how we can calculate the molecular weight of calf thymus DNA (CT-DNA) from estimation of the intrinsic viscosity at zero-shear rate. We use the small-volume (< 100 μL per sample), automatic VROC^® initium one plus viscometer to perform shear rate sweeps of CT-DNA samples at 25°C.

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