Viscosity of Cellular Suspensions

"In brief, we used the RheoSense to measure the viscosity of cellular suspensions. Our goal was to determine the dilution factor needed for the cell suspension to match a running buffer. As demonstrated in the graph this happens around a 10 fold sample dilution factor. Consistent sample viscosity will enable CytoVale to develop robust cellular assays for our core applications involving the high throughput mechanical measurement of cells. 

The RheoSense viscometer enabled us to quickly and accurately measure small aliqouts of sample which was necessary due to limited amounts of biological material. Additionally the low volume allowed us to gather many replicates of data in order to accurately assess biological variability between and across samples. This capability allowed us to have much more robust data collection and higher sample sizes for each point on the curve as compared to using traditional capillary viscometry. "

– Annie Jensen, Cytovale