Application Note - Viscosity as a Probe for DNA Melting Transition

DNA is a charged polymer that carries the genetic information for the development, maintenance, and reproduction of organisms. Upon increasing the temperature to its melting transition range, DNA undergoes denaturation into single strands, resulting in an increase in solution viscosity.

In this application note, we present temperature-sweep measurements (ramp up and ramp down in temperature) of calf-thymus DNA (CT-DNA) taken with the VROC^® initium one plus. We also perform a van’t Hoff analysis of rescaled melting temperature vs. concentration to predict thermodynamic parameters associated with DNA structural recovery. Extracting such parameters can be important in multiple applications to predict the sequence of DNA, like the ratio of G-C to A-T bonds, as well as its stability in different buffers and conditions. 

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