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Shear Rate Dependent Viscosity of Concentrated Protein Solutions

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We have previously demonstrated the sensitivity of the low shear limiting viscosity to changes in protein pair interactions induced by varying buffer composition.  However, characterizing concentrated protein solutions with only a single viscosity value is incomplete.  A thorough analysis must include an evaluation of the shear rate dependence to identify the onset and extent of non-Newtonian behavior.  Therefore, rate sweeps were performed on a subset of the concentrated Bovine γ-Globulin formulations with a range of low shear plateau values to assess the impact of the protein-protein interactions (PPI) on the full viscosity profile.

KEYWORDS:

  • Viscosity
  • Protein Protein Interactions (PPI)
  • Bovine Gamma Globulin (Bovine γ-Globulin)
  • Shear Rate Dependence
  • non-Newtonian 
  • Buffer

 

1. How sensitive is viscosity change to concentration?

Extremely sensitive! We have done some sample concentration analysis with glycerol and small % changes can impact the viscosity significantly. Here are some sample application notes!

- L-Arginine Viscosity

- Concentration Protein Solutions

2. If you can do low concentrations, can you do high concentration measurements?

Yep! Similar to this application note, we have a number of more application notes that focus on various concentrations including higher concentration viscosity solutions. Check them out: 

- High Concentration Protein Solutions

- Optimization of Protein Solutions

About the Viscometer

Learn more about the automated viscometer that compliments Clariti™, VROC® initium.

  • 26 µL of Sampleinitium (no bdg)
  • Sample Retrieval 
  • Sample Recovery
  • Measurement temperatures 4 - 70 ºC
  • Sample storage temperature
  • Intrinsic Viscosity
  • Injectability
VROC initium
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