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Scientist - Cell Culture

Application Note: Viscosity of F12 Cell Culture Media


Cell culture media is a complex fluid composed of nutrients required for cells to grow, proliferate, and differentiate. Media is specifically designed to maintain the ideal nutrients, hormones, pH, and osmotic environment necessary for cell growth. Different types of cells prefer different types of media. For example,
many mammalians cell lines are grown and maintained using Ham’s or F12 media, while insect cells are typically grown in Grace’s medium. Furthermore, some types of cells are best grown in mixtures of different media formulations such as combination medias comprised of DMEM and F12. Characterizing the differences between formulations is vital for developing new products and for maintaining quality control for existing product lines.

In this application note, we investigated the viscosity of F12 media formulations. We showed that filtering the media with a 0.2 μm filter does not impact media viscosity, there is not a difference in viscosity between purchased and homemade DMEM/F12 media, and the viscosity of DMEM/F12 is equivalent to the
DMEM component in the formulation.

1. How is viscosity relevant to cell culture media?

Cell culture media is a fluid filled with nutrients required for cells to grow. Many aspects are important to it including nutrients, hormones, pH, and osmotic environment for cell growth.  A change in viscosity of the cell culture media indicates changes in the media (whether nutrients are intentionally added) or can also indicate that your cell media has changed and it's time to analyze. 

2. Can RheoSense viscometers do Newtonian and non-Newtonian samples?

Yes! VROC technology can handle non-Newtonian samples by having direct control over shear rates! Newtonian samples are also easy to do! 

About the Viscometer

Learn more about the automated viscometer that compliments Clariti™, VROC® initium.

  • 26 µL of Sampleinitium (no bdg)
  • Sample Retrieval 
  • Sample Recovery
  • Measurement temperatures 4 - 70 ºC
  • Sample storage temperature
  • Intrinsic Viscosity
  • Injectability
Learn More