Accurately Measuring Viscosity with only 10 μL on m-VROC II
m-VROC® II is now able to measure viscosity with a sample volume as low as 10 μL. The next generation m-VROC® II with its advanced features, is an ideal viscometer for measuring precious and limited quantity biological fluids. The highlighted features include the following:
- Small quantity of sample for repeated tests; thanks to the microfluidics & the sample retrieval feature!
- Excellent accuracy, ±2%, and repeatability, ±0.5%
The plot here displays the viscosity results obtained for 150 mg/mL Bovine g-Globulin (BgG), representing a low viscosity sample, and 0.1 wt% high molecular weight Hyaluronic acid (HA), representing a high viscosity sample, at a fixed shear rate and temperature 25 ˚C.
The test was conducted as follows:
- 10 µL of sample was back-loaded into the 100 µL test syringe using a positive displacement pipette.
- The m-VROC II unit was equipped with a B05 chip to perform measurements on the sample with five repeats at a fixed shear rate, retrieving the sample when required.
- Chip Cleaning Station (CCS) performed automatic cleaning of the chip and reservoir at the end of the test with the preset buffer cleaning protocol.
We obtained a set of five viscosity measurements with just 10 µL of each sample, demonstrating the superior repeatability. The average viscosity was measured as 5.38 cP for 150 mg/mL BgG and 200.2 cP for 0.1 wt% HA. The low sample volume capability allows for fast and accurate screening of drug candidates in the early-stage drug development.