<img height="1" width="1" style="display:none;" alt="" src="https://dc.ads.linkedin.com/collect/?pid=510762&amp;fmt=gif">

 

 

   

 

 

Quantify Impact of
Buffer Additives On Protein Viscosity
 

Intrinsic Viscosity of Protein Solutions – Impact of Buffer Additives 

Are your buffer additives effective at controlling viscosity?

Buffer additives are often chosen specifically to modify the viscosity and stability of protein solutions.  It is, of course, practical to measure the viscosity of the fully formulated solution to predict its ability to perform.  However, a more detailed viscosity analysis can reveal the impact of the buffer components on both the individual protein behavior as well as the interaction between neighboring molecules.  Establishing such a knowledge base can provide guidance for future formulation activities and eliminate the need for guess work. 

The intrinsic viscosity protocol involves measurement of a concentration series in the dilute regime.  The analysis is simplified in the dilute limit since viscosity can be expressed as a series expansion in concentration.  The coefficients can then be used to quantify the individual protein contribution and the pair or protein-protein interactions (PPI).  The relevant equations and meaning of the extracted parameters are presented here.  Viscosity data for Bovine gamma globulin solutions in various buffers is used to illustrate the analysis procedure.    

 ————————————————————————————

Submit form to access full application note.

Key Words:

  • Viscosity
  • Protein Solutions
  • Bovine Gamma Globulin
  • Intrinsic Viscosity
  • Urea Sucrose
  • Arginine Hydrochloride
  • Huggins Coefficient
  • Hydrodynamic Radius

 

Check out our application notes!
Application Note Download Form

Banner Image.png

News & Articles


Cannabis Viscosity Webinar

The cannabis industry has been growing as regulations continue to change and the demand for cannabis infused products are increasing. We have been wor...

Not doing stability studies? Here's why you should.

Protein stability and understanding how your proteins will work is so important. It's so important that there are departments made for just the proces...

Question: Rheometer vs. VROC

I recently had a customer who asked how VROC® technology measures viscosity compared to a rheometer that measures shear stress and also control shear ...

Peptalk 2020 - Presentation

At the 2020 Peptalk Conference that took place in San Diego, our Principal Scientist, Dr. Stacey Elliott, gave a talk regarding a thorough investigati...

Google+