Sample Retrieval for Multiple Viscosity Measurements: 20 Different Shear Rates with 18 µL of Sample!

In addition to the low sample volume consumption, VROC® initium viscometer features sample retrieval, allowing to reuse a small quantity of sample for repeated tests. This functionality enables the characterization of samples over a wide range of shear rates with just about a tear drop! For this application brief, we tested a single 18 µL sample of Bovine Serum Albumin (BSA) 302 mg/mL in water at 20 different shear rates!

The test was conducted as follows:

  1. 25 µL of BSA (302 mg/ml in water) was loaded into an 11 mm vial with a 300 µL small-volume insert (Wheaton)
  2. The autosampler loaded 18 µL of the sample into the VROC® initium viscometer
  3. VROC® initium performed multiple shear rate measurements on the sample, retrieving the sample when required
  4. VROC® initium performed self-cleaning at the end of the test with preset protocols

Overall, 18 µL of BSA was consumed to test at 20 different shear rates, from 1066 to 2840 1/s. The BSA sample exhibits Newtonian behavior across the range tested (See Figure), with an average viscosity of 6.078cP at 25 oC, and a relative standard deviation of 0.51%!

Viscosity of 18 uL sample of 300 mg/ml BSA at 25 C

 Want to read more about automating your viscosity measurements? Visit our application library! ↓  

Download More Application Notes on Automating Your Viscosity Measurements with VROC initium!